Journal
NATURE PROTOCOLS
Volume 3, Issue 9, Pages 1527-1534Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2008.144
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Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NIGMS NIH HHS [P20 GM072033-03, P20 GM072033-01, P20 GM072033-04, P20 GM072033, P20 GM072033-03S1, P20 GM072033-02] Funding Source: Medline
- NINDS NIH HHS [R01 NS027177, R37 NS027177, R01 NS027177-18, R37 NS027177-19, R01 NS027177-15, R01 NS027177-17, R01 NS027177-16] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM072033] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R37NS027177, R01NS027177] Funding Source: NIH RePORTER
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The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin, respectively. Handling of toxic reagents such as arsenic trichloride is minimized so the synthesis can be carried out in a typical chemistry laboratory, usually taking about 2-3 d. A wide range of other biarsenical reagents and intermediates that also bind to tetracysteine-tagged (CysCysProGlyCysCys) proteins can be prepared similarly using this general procedure.
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