Journal
NATURE NEUROSCIENCE
Volume 17, Issue 6, Pages 884-889Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.3709
Keywords
-
Categories
Funding
- DARPA
- National Science Foundation [1134416]
- Stanford Graduate Fellowship
- Walter V. and Idun Berry Postdoctoral Fellowship
- Stanford University Bio-X Interdisciplinary Initiatives Project grant
- Stanford CNC Program
- Howard Hughes Medical Institute
- National Academy of Sciences Keck Futures Initiative
- Rita Allen Foundation
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1134416] Funding Source: National Science Foundation
Ask authors/readers for more resources
Accurate optical reporting of electrical activity in genetically defined neuronal populations is a long-standing goal in neuroscience. We developed Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential. ASAP1 demonstrated on and off kinetics of similar to 2 ms, reliably detected single action potentials and subthreshold potential changes, and tracked trains of action potential waveforms up to 200 Hz in single trials. With a favorable combination of brightness, dynamic range and speed, ASAP1 enables continuous monitoring of membrane potential in neurons at kilohertz frame rates using standard epifluorescence microscopy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available