Journal
NATURE NEUROSCIENCE
Volume 17, Issue 3, Pages 383-390Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.3646
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Funding
- SystemsX.ch (NeuroChoice)
- Swiss National Science Foundation [31003A_130694]
- Whitaker International Program
- DFG [EXC 257 NeuroCure]
- BBSRC [BB/H002383/2] Funding Source: UKRI
- MRC [G0400571] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H002383/2] Funding Source: researchfish
- Medical Research Council [G0400571] Funding Source: researchfish
- Swiss National Science Foundation (SNF) [31003A_130694] Funding Source: Swiss National Science Foundation (SNF)
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Recent evidence in vitro suggests that the tuft dendrites of pyramidal neurons are capable of evoking local NMDA receptor-dependent electrogenesis, so-called NMDA spikes. However, it has so far proved difficult to demonstrate their existence in vivo. Moreover, it is not clear whether NMDA spikes are relevant to the output of pyramidal neurons. We found that local NMDA spikes occurred in tuft dendrites of layer 2/3 pyramidal neurons both spontaneously and following sensory input, and had a large influence on the number of output action potentials. Using two-photon activation of an intracellular caged NMDA receptor antagonist (tc-MK801), we found that isolated NMDA spikes typically occurred in multiple branches simultaneously and that sensory stimulation substantially increased their probability. Our results demonstrate that NMDA receptors have a vital role in coupling the tuft region of the layer 2/3 pyramidal neuron to the cell body, enhancing the effectiveness of layer 1 input.
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