Journal
NATURE NEUROSCIENCE
Volume 15, Issue 8, Pages 1120-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.3142
Keywords
-
Categories
Funding
- Agence National de la Recherche (ANR, FORDOPA)
- Foundation pour la Recherche Medicale (Label Equipe FRM)
- Fondation de France
- European Commission
- Region PACA [CG06]
- ANR
- Vaincre la Mucoviscidose
- Association pour la Recherche sur le Cancer
- Institut National du Cancer
Ask authors/readers for more resources
In the postnatal and adult mouse forebrain, a mosaic of spatially separated neural stem cells along the lateral wall of the ventricles generates defined types of olfactory bulb neurons. To understand the mechanisms underlying the regionalization of the stem cell pool, we focused on the transcription factor Pax6, a determinant of the dopaminergic phenotype in this system. We found that, although Pax6 mRNA was transcribed widely along the ventricular walls, Pax6 protein was restricted to the dorsal aspect. This dorsal restriction was a result of inhibition of protein expression by miR-7a, a microRNA (miRNA) that was expressed in a gradient opposing Pax6. In vivo inhibition of miR-7a in Pax6-negative regions of the lateral wall induced Pax6 protein expression and increased dopaminergic neurons in the olfactory bulb. These findings establish miRNA-mediated fine-tuning of protein expression as a mechanism for controlling neuronal stem cell diversity and, consequently, neuronal phenotype.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available