Journal
NATURE NEUROSCIENCE
Volume 12, Issue 10, Pages 1300-U124Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.2395
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Funding
- Canadian Institute of Health Research [MOP68939, MOP89720, MOP15514]
- US National Institutes of Health [NS048956]
- Canadian Stroke Networks
- Krembil Seed fund
- Heart and Stroke Foundation of Canada Focus
- Grants-in-Aid for Scientific Research [20249015] Funding Source: KAKEN
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Cardiac arrest victims may experience transient brain hypoperfusion leading to delayed death of hippocampal CA1 neurons and cognitive impairment. We prevented this in adult rats by inhibiting the expression of transient receptor potential melastatin 7 (TRPM7), a transient receptor potential channel that is essential for embryonic development, is necessary for cell survival and trace ion homeostasis in vitro, and whose global deletion in mice is lethal. TRPM7 was suppressed in CA1 neurons by intrahippocampal injections of viral vectors bearing shRNA specific for TRPM7. This had no ill effect on animal survival, neuronal and dendritic morphology, neuronal excitability, or synaptic plasticity, as exemplified by robust long-term potentiation (LTP). However, TRPM7 suppression made neurons resistant to ischemic death after brain ischemia and preserved neuronal morphology and function. Also, it prevented ischemia-induced deficits in LTP and preserved performance in fear-associated and spatial-navigational memory tasks. Thus, regional suppression of TRPM7 is feasible, well tolerated and inhibits delayed neuronal death in vivo.
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