Journal
NATURE NANOTECHNOLOGY
Volume 9, Issue 7, Pages 537-541Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2014.102
Keywords
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Funding
- National Institute of General Medical Sciences [GM-29554]
- National Science Foundation [CMMI-1120890, CCF-1117210]
- MURI from the Army Research Office [W911NF-11-1-0024]
- Office of Naval Research [N000141110729, N000140911118]
- Australian Nanotechnology Network Overseas Travel Fellowship
- Melbourne Abroad Travelling Scholarship
- Bio21 Institute
- Particulate Fluids Processing Centre
- ARC Dairy Innovation Hub
- US Department of Energy, Office of Basic Energy Sciences [DE-AC02-98CH10886]
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1120890] Funding Source: National Science Foundation
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1117210] Funding Source: National Science Foundation
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Amyloid fibrils are ordered, insoluble protein aggregates that are associated with neurodegenerative conditions such as Alzheimer's disease(1). The fibrils have a common rod-like core structure, formed from an elongated stack of a-strands, and have a rigidity similar to that of silk (Young's modulus of 0.2-14 GPa)(2). They also exhibit high thermal and chemical stability(3) and can be assembled in vitro from short synthetic non-disease-related peptides(4,5). As a result, they are of significant interest in the development of self-assembled materials for bionanotechnology applications(6). Synthetic DNA molecules have previously been used to form intricate structures and organize other materials such as metal nanoparticles(7,8) and could in principle be used to nucleate and organize amyloid fibrils. Here, we show that DNA origami nanotubes can sheathe amyloid fibrils formed within them. The fibrils are built by modifying the synthetic peptide fragment corresponding to residues 105-115 of the amyloidogenic protein transthyretin(9) and a DNA origami(10) construct is used to form 20-helix DNA nanotubes with sufficient space for the fibrils inside. Once formed, the fibril-filled nanotubes can be organized onto predefined two-dimensional platforms via DNA-DNA hybridization interactions.
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