Journal
NATURE NANOTECHNOLOGY
Volume 7, Issue 12, Pages 821-824Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2012.186
Keywords
-
Funding
- Engineering and Physical Sciences Research Council (EPSRC)
- European Research Council (ERC)
- European Community
- EPSRC [EP/E007627/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/E007627/1] Funding Source: researchfish
Ask authors/readers for more resources
In resource-constrained countries, affordable methodologies for the detection of disease biomarkers at ultralow concentrations can potentially improve the standard of living(1,2). However, current strategies for ultrasensitive detection often require sophisticated instruments that may not be available in laboratories with fewer resources(3-11). Here, we circumvent this problem by introducing a signal generation mechanism for biosensing that enables the detection of a few molecules of analyte with the naked eye. The enzyme label of an enzyme-linked immunosorbent assay (ELISA) controls the growth of gold nanoparticles and generates coloured solutions with distinct tonality when the analyte is present. Prostate specific antigen (PSA) and HIV-1 capsid antigen p24 were detected in whole serum at the ultralow concentration of 1x10(-18) g ml(-1). p24 was also detected with the naked eye in the sera of HIV-infected patients showing viral loads undetectable by a gold standard nucleic acid-based test.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available