Journal
NATURE NANOTECHNOLOGY
Volume 6, Issue 2, Pages 125-131Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nnano.2010.275
Keywords
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Funding
- National Science Foundation [ENG-0707748, CHE-0641523]
- New York State Office of Science, Technology, and Academic Research (NYSTAR)
- Office of Naval Research [N00014-09-01-0250, N00014-09-1-1117]
- National Institutes of Health [R33-HG003089]
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Single-molecule measurements of biomolecules can provide information about the molecular interactions and kinetics that are hidden in ensemble measurements. However, there is a requirement for techniques with improved sensitivity and time resolution for use in exploring biomolecular systems with fast dynamics. Here, we report the detection of DNA hybridization at the single-molecule level using a carbon nanotube field-effect transistor. By covalently attaching a single-stranded probe DNA sequence to a point defect in a carbon nanotube, we are able to measure two-level fluctuations in the conductance of the nanotube in the presence of a complementary DNA target. The kinetics of the system are studied as a function of temperature, allowing the measurement of rate constants, melting curves and activation energies for different sequences and target concentrations. The kinetics demonstrate non-Arrhenius behaviour, in agreement with DNA hybridization experiments using fluorescence correlation spectroscopy. This technique is label-free and could be used to probe single-molecule dynamics at microsecond timescales.
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