Journal
NATURE METHODS
Volume 15, Issue 10, Pages 777-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0140-x
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Funding
- European Research Council (ERC) [AdG 340227]
- European Union's Horizon 2020 Framework Programme for Research and Innovation [665667]
- National Centre for Competence in Research (NCCR) Chemical Biology
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Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.
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