Journal
NATURE METHODS
Volume 15, Issue 9, Pages 741-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0107-y
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Funding
- Division of Intramural Research of NHLBI
- Division of Intramural Research of NIH
- Division of Intramural Research of CCR, NCI, NIH
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Long-range chromatin interactions play critical roles in genome organization and regulation of transcription. We now report transposase-mediated analysis of chromatin looping (Trac-looping) for simultaneous detection of multiscale genome-wide chromatin interactions among regulatory elements and chromatin accessibility. With this technique, a bivalent oligonucleotide linker is inserted between two interacting regions such that the chromatin interactions are captured without prior chromatin fragmentation and proximity-based ligation. Application of Trac-looping to human CD4(+) T cells revealed substantial reorganization of enhancer-promoter interactions associated with changes in gene expression after T cell receptor stimulation.
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