Journal
NATURE METHODS
Volume 11, Issue 7, Pages 734-U174Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2962
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Funding
- X-ray Free Electron Laser Priority Strategy Program (The Ministry of Education, Culture, Sports, Science and Technology in Japan (MEXT))
- JST/CREST
- MEXT/Japan Society for the Promotion of Science (JSPS) [24000018]
- Japan Synchrotron Radiation Research Institute (JASRI) [2012A8011, 2012B8040, 2013A8047, 2013B8052]
- Grants-in-Aid for Scientific Research [22370060, 26291033, 25109540, 24000018, 26104532] Funding Source: KAKEN
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We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-angstrom radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
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