Journal
NATURE METHODS
Volume 12, Issue 1, Pages 61-63Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3172
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Funding
- Japan Synchrotron Radiation Research Institute (JASRI) [2012B8036, 2013A8039, 2013A8040, 2013B8044, 2013B8045, 2014A8032]
- RIKEN
- X-ray Free-Electron Laser Priority Strategy Program (MEXT)
- Research Acceleration Program of Japan Science and Technology Agency
- JST-ERATO Murata Lipid Active Structure Project
- Grants-in-Aid for Scientific Research [26560448] Funding Source: KAKEN
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Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3. under ambient conditions at a resolution of or finer than 2 angstrom.
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