Journal
NATURE METHODS
Volume 11, Issue 6, Pages 663-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2938
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Funding
- Wyss Institute for Biologically Inspired Engineering at Harvard University
- Defense Advanced Research Projects Agency [W911NF-12-2-0036]
- US Food and Drug Administration (FDA) [HHSF223201310079C]
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Current in vitro hematopoiesis models fail to demonstrate the cellular diversity and complex functions of living bone marrow; hence, most translational studies relevant to the hematologic system are conducted in live animals. Here we describe a method for fabricating 'bone marrow-on-a-chip' that permits culture of living marrow with a functional hematopoietic niche in vitro by first engineering new bone in vivo, removing it whole and perfusing it with culture medium in a microfluidic device. The engineered bone marrow (eBM) retains hematopoietic stem and progenitor cells in normal in vivo-like proportions for at least 1 week in culture. eBM models organ-level marrow toxicity responses and protective effects of radiation countermeasure drugs, whereas conventional bone marrow culture methods do not. This biomimetic microdevice offers a new approach for analysis of drug responses and toxicities in bone marrow as well as for study of hematopoiesis and hematologic diseases in vitro.
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