Journal
NATURE METHODS
Volume 11, Issue 3, Pages 291-U345Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2840
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Funding
- Japan Society for the Promotion of Science Postdoctoral Fellowship for Research Abroad
- Uehara Memorial Foundation Research Fellowship
- California Institute of Regenerative Medicine [TG2-01160]
- American Cancer Society [PF-13-295-01-TBG]
- US National Heart, Lung, and Blood Institute, National Institutes of Health [U01-HL100406, U01-GM09614, R01-HL108677, U01-HL098179, R01-HL060664]
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Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.
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