Journal
NATURE METHODS
Volume 11, Issue 2, Pages 190-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.2804
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Funding
- PhRMA foundation
- US National Institutes of Health (NIH) [R01 GM083030]
- McKnight Foundation Technology Innovations Award
- NIH [DP004117]
- Penn Genome Frontiers Institute
- Pennsylvania Department of Health
- [U01MH098953]
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Transcriptome profiling of single cells resident in their natural microenvironment depends upon RNA capture methods that are both noninvasive and spatially precise. We engineered a transcriptome in vivo analysis (TIVA) tag, which upon photoactivation enables mRNRNA capture from single cells in live tissue. Using the TIVA tag in combination with RNA sequencing (RNA-seq), we analyzed transcriptome variance among single neurons in culture and in mouse and human tissue in vivo. Our data showed that the tissue microenvironment shapes the transcriptomic landscape of individual cells. The TIVA methodology is, to our knowledge, the first noninvasive approach for capturing mRNA from live single cells in their natural microenvironment.
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