Journal
NATURE METHODS
Volume 11, Issue 9, Pages 919-922Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3037
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Funding
- Max Planck Society
- Human Frontier Science Program [CDA 00063/2010-C]
- Boehringer Ingelheim Fonds
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The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
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