Journal
NATURE METHODS
Volume 11, Issue 3, Pages 253-266Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2843
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Funding
- Agency for Innovation by Science and Technology (IWT, Belgium)
- Ghent University Special Research Fund
- Fund for Scientific Research Flanders (FWO, Belgium)
- US National Institutes of Health [R15-GM094713]
- Maine Technology Institute [MTAF 1106, 2061]
- Italian Programmi di Ricerca di Rilevante Interesse Nazionale [2010JFYFY2-002]
- [PF7-EU 280804-2 LANIR CP-TP]
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Methods based on single-molecule localization and photophysics have brought nanoscale imaging with visible light into reach. This has enabled single-particle tracking applications for studying the dynamics of molecules and nanoparticles and contributed to the recent revolution in super-resolution localization microscopy techniques. Crucial to the optimization of such methods are the precision and accuracy with which single fluorophores and nanoparticles can be localized. We present a lucid synthesis of the developments on this localization precision and accuracy and their practical implications in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy.
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