Journal
NATURE METHODS
Volume 11, Issue 5, Pages 549-551Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2910
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Funding
- US National Institutes of Health (NIH)
- National Cancer Institute Physical Sciences Oncology Center at Massachusetts Institute of Technology [U54CA143874]
- NIH Pioneer award [8 DP1 'CA174420-05]
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) Vici award
- NIH [HD 045022, R37CA084198]
- Vertex Scholarship, a US National Science Foundation Graduate Research Fellowship
- Jerome and Florence Brill Graduate Student Fellowship
- Koch Institute for Integrative Cancer Research Graduate Fellowship
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We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is broadly applicable to profiling transcriptionally distinct cellular states without requiring antibodies or transgenic fluorescent proteins.
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