Journal
NATURE METHODS
Volume 11, Issue 3, Pages 313-U292Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2835
Keywords
-
Categories
Funding
- US National Institutes of Health (NIH) Director's New Innovator Award [1DP2OD007292]
- NIH Transformative Research Award [1R01EB018659]
- NIH [5R21HD072481]
- Office of Naval Research (ONR) Young Investigator Program Award [N000141110914]
- ONR [N000141010827, N000141310593]
- US National Science Foundation (NSF) Faculty Early Career Development Award [CCF1054898]
- NSF [CCF1162459]
- Wyss Institute for Biologically Engineering Faculty Startup Fund
- NIH Director's New Innovator Award [1DP2OD004641]
- Wyss Institute for Biologically Inspired Engineering Faculty Award
- Alexander von Humboldt-Foundation through a Feodor-Lynen Fellowship
- Howard Hughes Medical Institute International Student Research Fellowships
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [1317694] Funding Source: National Science Foundation
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1054898] Funding Source: National Science Foundation
Ask authors/readers for more resources
Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species remains challenging. Here we use the transient binding of short fluorescently labeled oligonucleotides (DNA-PAINT, a variation of point accumulation for imaging in nanoscale topography) for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution in vitro on synthetic DNA structures. We also report a multiplexing approach (Exchange-PAINT) that allows sequential imaging of multiple targets using only a single dye and a single laser source. We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional (2D) imaging and three-color 3D imaging of proteins in fixed cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available