Journal
NATURE METHODS
Volume 11, Issue 4, Pages 399-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2857
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Funding
- 973 program [2010CB945101, 2011CB944301]
- Wellcome Trust
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Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations.
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