Journal
NATURE METHODS
Volume 11, Issue 9, Pages 923-926Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3067
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Funding
- Swedish Science Research Council (VR)
- Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
- Swedish Strategic Research Foundation (SSF)
- Knut and Alice Wallenberg Foundation
- US National Science Foundation (NSF) and its bioXFEL Science and Technology Center [NSF 1231306]
- US National Institute of Health (NIH)
- DOE Office of Basic Energy Sciences
- Hamburg Ministry of Science and Research
- Joachim Herz Stiftung
- Deutsche Forschungsgemeinschaft (DFG)
- German Federal Ministry for Education and Research (BMBF),
- European Union
- Academy of Finland
- Max Planck Society
- Danish National Research Foundations Centre for Molecular Movie
- DANSCATT
- UCOP Lab [118036]
- LLNL [12-ERD-031]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1021557] Funding Source: National Science Foundation
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We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.
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