Journal
NATURE METHODS
Volume 11, Issue 5, Pages 529-534Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2889
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Funding
- US National Institutes of Health (NIH) Office of Research Infrastructure Programs [P40 0D010440]
- Carlsberg Foundation
- NIH [1R01GM095817]
- US National Science Foundation grant NSF [IOS-0920069]
- Howard Hughes Medical Institute
- Max Planck Society (MPG) Initiative BAC TransgeneOmics
- NIH ModENCODE project
- Canadian Institute for Health Research
- Canadian Institute for Advanced Research
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We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon.(miniMos) is 550 bp Long and inserts DNA into the genome at high frequency (similar to 60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the minifilos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lac0 insertions to track genome position.
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