Journal
NATURE METHODS
Volume 11, Issue 5, Pages 521-526Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.2894
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The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first synthetic cell was created 4 years ago. An outcome of this work has been an extraordinary set of tools for synthesizing, assembling, engineering and transplanting whole bacterial genomes. Technical progress, options and applications for bacterial genome design, assembly and activation are discussed.
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