Journal
NATURE METHODS
Volume 11, Issue 7, Pages 731-U168Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2972
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Funding
- Swiss National Science Foundation
- National Centre of Competence of Research (NCCR) Chemical Biology
- Korber foundation through the European Science Prize
- European Community's [241548, 258068]
- European Research Council (ERC) [281198]
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We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.
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