Journal
NATURE METHODS
Volume 11, Issue 6, Pages 633-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2940
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Funding
- National Research Foundation of Korea Stem Cell Program [2011-0019509]
- Intelligent Synthetic Biology Center of Global Frontier Project [2011-0031955]
- Korea Advanced Institute of Science and Technology Future Systems Healthcare Project - Ministry of Science, Information and Communication Technology & Future Planning in Korea
- National Research Foundation of Korea [2011-0019509] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.
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