Journal
NATURE METHODS
Volume 10, Issue 8, Pages 741-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2532
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Funding
- US National Institutes of Health Early Independence Award [1DP5OD009153]
- Harvard University
- National Institutes of Health [R01GM072551]
- National Human Genome Research Institute Center of Excellence in Genome Sciences award
- Ralph Ellison/American Federation for Aging Research postdoctoral fellowship
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We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
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