4.8 Article

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

Journal

NATURE METHODS
Volume 10, Issue 5, Pages 407-+

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2413

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Funding

  1. NIDDK NIH HHS [R01 DK043701, P30 DK079312] Funding Source: Medline

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We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

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