Journal
NATURE METHODS
Volume 10, Issue 6, Pages 508-513Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2481
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Funding
- US National Institutes of Health Director's Transformative Research Award [TR01]
- National Institute of Mental Health
- National Science Foundation
- Simons Foundation
- President and Provost of Stanford University
- Howard Hughes Medical Institute
- National Institute on Drug Abuse
- Defense Advanced Research Projects Agency Reorganization and Plasticity to Accelerate Injury Recovery program
- Wiegers Foundation
- Snyder Foundation
- Reeves Foundation
- Gatsby Foundation
- Yu Foundation
- Burroughs Wellcome Fund Career Award at the Scientific Interface
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1247950] Funding Source: National Science Foundation
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With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. T This process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. H Here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain-mapping studies.
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