Journal
NATURE METHODS
Volume 10, Issue 4, Pages 329-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2374
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Funding
- 973 program [2012CB945101, 2011CBA01000]
- National Natural Science Foundation of China [31110103904, 30871418, 30730056]
- US National Institutes of Health [DK54508, DK084349]
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We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator-like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.
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