Journal
NATURE METHODS
Volume 10, Issue 11, Pages 1099-1101Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2644
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Funding
- Spanish Ministerio de Ciencia e Innovacion-Ministerio de Economia y Competitividad
- Centro de Desarrollo Tecnologico
- Consejo Superior de Investigaciones Cientificas [BIO2009-10576, IDC-2010-1173, BFU2012-35367, BFU2012-32847]
- Generalitat de Catalunya [2009SGR-1036]
- VW-Stiftung Niedersachsenprofessur
- ICREA Funding Source: Custom
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We describe an algorithm for phasing protein crystal X-ray diffraction data that identifies, retrieves, refines and exploits general tertiary structural information from small fragments available in the Protein Data Bank. The algorithm successfully phased, through unspecific molecular replacement combined with density modification, all-helical, mixed alpha-beta, and all-beta protein structures. The method is available as a software implementation: Borges.
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