4.8 Article

Quantitative estimation of activity and quality for collections of functional genetic elements

Journal

NATURE METHODS
Volume 10, Issue 4, Pages 347-+

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2403

Keywords

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Funding

  1. National Science Foundation [EEC 0946510]
  2. Human Frontier Science Program [LT000873/2011-l]
  3. Bettencourt Schueller foundation
  4. Synthetic Biology Engineering Research Center under National Science Foundation [04-570/0540879]
  5. Portuguese Fundacao para a Ciencia e a Tecnologia [SFRH/BD/47819/2008]
  6. Office of Science, Office of Biological and Environmental Research of the US Department of Energy [DE-AC02-05CH11231]
  7. Fundação para a Ciência e a Tecnologia [SFRH/BD/47819/2008] Funding Source: FCT

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The practice of engineering biology now depends on the ad hoc reuse of genetic elements whose precise activities vary across changing contexts. Methods are lacking for researchers to affordably coordinate the quantification and analysis of part performance across varied environments, as needed to identify, evaluate and improve problematic part types. We developed an easy-to-use analysis of variance (ANOVA) framework for quantifying the performance of genetic elements. For proof of concept, we assembled and analyzed combinations of prokaryotic transcription and translation initiation elements in Escherichia coli. We determined how estimation of part activity relates to the number of unique element combinations tested, and we show how to estimate expected ensemble-wide part activity from just one or two measurements. We propose a new statistic, biomolecular part 'quality', for tracking quantitative variation in part performance across changing contexts.

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