Journal
NATURE METHODS
Volume 11, Issue 2, Pages 167-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2767
Keywords
-
Categories
Funding
- Deutsche Forschungsgemeinschaft [INST 371/23-1 FUGG]
- BMBF (e:Bio Express2Present) [0316179C]
- Forschungszentrum Immunologie (FZI)
- Naturwissenschaftlich-Medizinische Forschungszentrum (NMFZ)
- Focus Program Translational Neurosciences (FTN) of the Johannes Gutenberg University Mainz
Ask authors/readers for more resources
We present a data-independent acquisition mass spectrometry method, ultradefinition (UD) MSE. This approach utilizes ion mobility drift time-specific collision-energy profiles to enhance precursor fragmentation efficiency over current MSE and high-definition (HD) MSE data-independent acquisition techniques. UDMSE provided high reproducibility and substantially improved proteome coverage of the HeLa cell proteome compared to previous implementations of MSE, and it also outperformed a state-of-the-art data-dependent acquisition workflow. Additionally, we report a software tool, ISOQuant, for processing label-free quantitative UDMSE data.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available