Journal
NATURE METHODS
Volume 10, Issue 8, Pages 737-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2556
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Funding
- Swedish Research Council
- German Research Foundation (DFG)-Center of Nanoscale Microscopy and Molecular Physiology of the Brain
- German Ministry of Research (BMBF)
- Korber Foundation, Hamburg, through the European Science Prize
- Medical Research Council [MC_UU_12010/9, MR/K01577X/1] Funding Source: researchfish
- MRC [MR/K01577X/1, MC_UU_12010/9] Funding Source: UKRI
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We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 mm x 100 mm-sized fields of view in <1 s using 116,000 such doughnuts.
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