Journal
NATURE METHODS
Volume 10, Issue 10, Pages 973-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.2600
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Funding
- US National Institutes of Health (NIH) [DP20D008586]
- National Science Foundation (NSF) Faculty Early Career Development (CAREER) Award [CBET-1151035]
- NIH [R03AR061042, U54HG004563, EB015300, HL109442, R01AR48852, T32GM008555]
- American Heart Association Scientist Development Grant [10SDG3060033]
- NSF
- American Heart Association
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1151035] Funding Source: National Science Foundation
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Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
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