Journal
NATURE METHODS
Volume 10, Issue 5, Pages 438-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2437
Keywords
-
Categories
Funding
- American Recovery and Reinvestment Act (ARRA)
- US National Institutes of Health (NIH) [R01 GM065918]
- NIH [R43 NS080407]
- Stem Cell Engineering Center at Georgia Institute of Technology
- Sloan Foundation
- National Science Foundation (NSF) [DBI-0649833]
- ARRA sub-award [RC1CA144825]
- NSF [CMMI-1129611]
- Georgia Tech Emory Center for Regenerative Medicine
Ask authors/readers for more resources
We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over similar to 10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available