Journal
NATURE METHODS
Volume 11, Issue 2, Pages 163-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2772
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Funding
- Swedish Research Council (STARGET)
- European Research Council [261063]
- Swedish Cancer Society
- Swedish Research Council
- Ragnar Soderberg Foundation
- European Research Council (ERC) [261063] Funding Source: European Research Council (ERC)
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Single-cell RNA sequencing (RNA-seq) is a powerful tool to reveal cellular heterogeneity, discover new cell types and characterize tumor microevolution. However, losses in cDNA synthesis and bias in cDNA amplification lead to severe quantitative errors. We show that molecular labels-random sequences that label individual molecules-can nearly eliminate amplification noise, and that microfluidic sample preparation and optimized reagents produce a fivefold improvement in mRNA capture efficiency.
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