Journal
NATURE METHODS
Volume 10, Issue 8, Pages 744-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2528
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Funding
- US National Institutes of Health [R01 GM103551, P41 GM103533, F31 AG037265]
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In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five-to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.
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