Journal
NATURE METHODS
Volume 11, Issue 1, Pages 51-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2736
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Funding
- Stanford University School of Medicine
- Stanford Graduate Fellowship
- US National Institutes of Health [GM102484]
- Ellison Medical Foundation
- United States-Israel Binational Science Foundation
- Edward Mallinckrodt, Jr. Foundation
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [T32HG000044] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM102484] Funding Source: NIH RePORTER
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We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels and to accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq for studying allelic variations in the transcriptome.
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