Journal
NATURE METHODS
Volume 10, Issue 4, Pages 343-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.2401
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Funding
- Wellcome Trust [WT094296MA]
- EU-FP7 'Sybilla' [201106]
- Deutsche Forschungsgemeinschaft Emmy Noether grant [DI1512/1-1]
- Royal Society
- Biotechnology and Biological Sciences Research Council
- Marie Curie Intra European Fellowship
- Rhodes scholarship
- BBSRC [BB/E004350/1] Funding Source: UKRI
- EPSRC [EP/E000614/1, EP/I500200/1, EP/G026688/1] Funding Source: UKRI
- MRC [G0501068] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [EGA17763, BB/E004350/1, BB/C510824/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [GR/T26542/01, EP/D023343/1, EP/I500200/1, EP/G026688/1, EP/E000614/1, EP/D023335/1] Funding Source: researchfish
- Medical Research Council [G0501068] Funding Source: researchfish
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Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
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