Journal
NATURE METHODS
Volume 10, Issue 8, Pages 747-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2522
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Funding
- US National Institutes of Health [GM085530, HG002096-11]
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We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.
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