Journal
NATURE METHODS
Volume 9, Issue 2, Pages 185-188Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1841
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Funding
- US National Institutes of Health
- Howard Hughes Medical Institute [43667]
- Gatsby Charitable Foundation
- Howard Hughes Medical Institute
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By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.
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