Journal
NATURE METHODS
Volume 9, Issue 12, Pages 1192-U85Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2225
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Funding
- Department of Defense National Defense Science & Engineering Graduate Fellowship
- US National Institutes of Health [R01-HG004361, R01GM29832]
- Howard Hughes Medical Institute
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We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
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