Journal
NATURE METHODS
Volume 9, Issue 7, Pages 730-U304Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.2064
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Funding
- EMBL
- EMBL Interdisciplinary Postdocs Program (EIPOD)
- Center for Modelling and Simulation in the Biosciences (BIOMS)
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We present a multiview selective-plane illumination microscope (MuVi-SPIMIM), comprising two detection and illumination objective lenses, that allows rapid in toto fluorescence imaging of biological specimens with subcellular resolution. The fixed geometrical arrangement of the imaging branches enables multiview data fusion in real time. The high speed of MuVi-SPIMIM allows faithful tracking of nuclei and cell shape changes, which we demonstrate through in toto imaging of the embryonic development of Drosophila melanogaster.
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