Journal
NATURE METHODS
Volume 9, Issue 12, Pages 1202-U103Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2249
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Funding
- HHMI
- Kavli Institute for Brain Science
- National Eye Institute
- Keck Foundation
- Deutsche Forschungsgemeinschaft (DFG) [HI 1728/1-1]
- Department of Defense Multidisciplinary University Research Initiative Program
- US National Institute of Mental Health
- US National Institutes of Health
- California Institute for Regenerative Medicine
- Gatsby Foundation
- Defense Advanced Research Projects Agency
- Direct For Computer & Info Scie & Enginr
- Div Of Information & Intelligent Systems [0904353] Funding Source: National Science Foundation
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We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.
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