Journal
NATURE METHODS
Volume 9, Issue 4, Pages 363-U66Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1898
Keywords
-
Categories
Funding
- Harvard Clinical and Translational Science Center
- US National Center for Research Resources
- Sanofi-Aventis
- US National Institutes of Health [GM66492]
- [1 UL1 RR025758-01]
Ask authors/readers for more resources
Because off-target effects hamper interpretation and validation of RNAi screen data, we developed a bioinformatics method, genome-wide enrichment of seed sequence matches (GESS), to identify candidate off-targeted transcripts in primary screening data. GESS analysis revealed a prominent off-targeted transcript in several screens, including MAD2 (MAD2L1) in a screen for genes required for the spindle assembly checkpoint. GESS analysis results can enhance the validation rate in RNAi screens.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
