Journal
NATURE METHODS
Volume 9, Issue 6, Pages 591-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1971
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Funding
- US National Science Foundation (SynBERC) [SA5283-11210]
- Department of Energy (Genome to Life Center) [DE-FG02-03ER6344]
- Wyss Institute for Biologically Inspired Engineering
- SSAC [PJ008109]
- Rural Development Administration
- Intelligent Synthetic Biology Center
- Ministry of Education, Science and Technology, Korea [2011-0031956]
- Wyss Institute
- US National Institutes of Health [DP5OD009172]
- National Research Foundation of Korea [2012-0000391]
- National Research Foundation of Korea [2011-0012414, 2011-0031955] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [PJ009034012012] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Multiplex automated genome engineering (MAGE) uses short oligonucleotides to scarlessly modify genomes; however, insertions >10 bases are still inefficient but can be improved substantially by selection of highly modified chromosomes. Here we describe 'coselection' MAGE (CoS-MAGE) to optimize biosynthesis of aromatic amino acid derivatives by combinatorially inserting multiple T7 promoters simultaneously into 12 genomic operons. Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks.
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