Journal
NATURE METHODS
Volume 9, Issue 7, Pages 721-U286Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1978
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Funding
- US National Institutes of Health [1R21EB012700-01 A1]
- UCSF
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In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s.
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