Journal
NATURE METHODS
Volume 9, Issue 8, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2077
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Funding
- Breast Cancer Research Program Department of Defense Era of Hope grant [W81XWH-05-1-0330]
- US National Institutes of Health (NIH)/National Cancer Institute (NCI) [1U54CA163155-01]
- NCI [U54CA143836-01]
- NIH/NCI [R01 CA138818-01A1]
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Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton as well as the temporal evolution, three-dimensional architecture and nanoscale dynamics of focal adhesion complexes.
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