Journal
NATURE METHODS
Volume 9, Issue 12, Pages 1189-U81Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2211
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Funding
- Office of Science, Office of Basic Energy Sciences, of the US Department of Energy [DE-AC02-05CH11231]
- US National Institutes of Health Research Grant [GM21841]
- King Abdulaziz University
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We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.
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