Journal
NATURE METHODS
Volume 9, Issue 3, Pages 270-U69Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1871
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Funding
- US National Institutes of Health [R01 DA025779, R01 GM097253]
- California Institute of Regenerative Medicine
- Royal Thai Government
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Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
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